percentage_feature_set

Adds a metadata variable that shows the perentage of a cell’s data that originates from features that match a regex.

module

Inputs

Channel nameDescriptionType
optA map of task-specific variables.map
tagA unique identifier to use in the tag directive.string
assayThe name of the assay from which percentages should be calculated.string
feature_sets.yamlYAML-formatted file of gene feature(s) or regular expression(s) where the YAML key dictates output column name.file
seurat.rdsA Seurat object that contains features listed in feature_sets.yaml.file

Outputs

Emission nameDescriptionTypeFilename pattern
optA map of task-specific variables.map
taskYAML-formatted file of task parameters and software versions used by the task.filetask.yaml
seuratA Seurat object written to RDS file with additional metadata varaibles for feature(s) listed in feature_sets.yaml. This may change to provide a data frame that can be collated with other similar results.fileseurat.rds

Tools

r

R is a free software environment for statistical computing and graphics.

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seurat

Seurat is an R package designed for QC, analysis, and exploration of single-cell RNA-seq data. Seurat aims to enable users to identify and interpret sources of heterogeneity from single-cell transcriptomic measurements, and to integrate diverse types of single-cell data.

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