make_assay

Writes assay objects as RDS files for a specified assay type.

module

Inputs

Channel nameDescriptionType
optA map of task-specific variables.map
tagA unique identifier to use in the tag directive.string
feature_typeType of feature in the counts matrices from which an assay should be created. This should be one of the feature types in the features.tsv.gz file (column 3), for example “Gene Expresssion”, “Peaks”, etc.string
counts_matrices.rdsThe RDS object that contains a list of the counts matrices for the dataset.file

Outputs

Emission nameDescriptionTypeFilename pattern
optA map of task-specific variables.map
taskYAML-formatted file of task parameters and software versions used by the task.filetask.yaml
assayRDS file of the assay object created from the counts matrix for the feature type.fileassay.rds

Tools

r

R is a free software environment for statistical computing and graphics.

Homepage Documentation

seurat

Seurat is an R package designed for QC, analysis, and exploration of single-cell RNA-seq data. Seurat aims to enable users to identify and interpret sources of heterogeneity from single-cell transcriptomic measurements, and to integrate diverse types of single-cell data.

DOI Homepage Documentation Source